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Vita Bee Health Global Honeybee Health Experts

B401: Wax Moth Control

B401 BottleB401 (also known as Certan) is a preventative treatment that controls wax moth. It is a safe and environmentally friendly product based on a concentrated solution of Bacillus thuringiensis, a micro-organism.

B401 is used after the honey harvest, when the frames are stored and kills young wax moth larvae. It must therefore be used before a wax moth infestation. A single application will provide very high efficacy against wax moth right up through to the following season. B401 comes in 120ml or 1 litre containers and is available from Vita distributors.


Key Facts

  • B401 is a concentrated solution of Bacillus thuringiensis subspecies aizawai, a micro-organism, harmless to man & honeybee alike.
  • 100 % efficacy against the larvae of the wax moth (Galleria mellonella).
  • A 100% biological product that leaves no residue in wax or honey, does not alter the taste of honey, and is environmentally friendly.
  • Is suitable for organic farming in accordance with Council Regulation EEC No 2092/91 modified on 24th June 1991.
  • Comes in 120ml or 1 litre containers and is available from Vita distributors.

How to use B401 biological wax moth control


  • B401 is made of spores from Bacillus thuringiensis and must be diluted in water to 5% (i.e. 1 volume of B401 for 19 volumes of water).
  • Shake the bottle of B401 vigorously before adding the required amount to the corresponding water volume and mixing.
  • Once diluted, the solution must be used the same day. Before mixing, calculate the amount of product required to treat the desired number of frames.


For up to 100% wax moth control, apply 1.5ml of solution per 10 centimetres squared of comb, on both sides of the frame. For example, one 120ml B401 bottle will treat 50-70 Dadant brood frames or 10-15 Dadant supers or 70-100 Langstroth brood frames.

Applying B401

  • Shake the bottle before use.
  • Apply evenly with a sprayer over both sides of the frame.
  • Allow the frames to dry in an aired room before storing.

When to use B401

B401-beeB401 should be used after the honey harvest, when the frames are stored. Protection will be effective right through to the following season.

B401 can also be used to protect decoy or empty hives.

B401 kills only young wax moth larvae and must be used as a preventative before the combs are infested.

How to store B401

B401 should be stored at a temperature between 5°C and 20°C. If stored in a cool place (under 12°C) and in its well-sealed original bottle, B401 can be kept for several years.

Safety of B401

Unlike paradichlorobenzene, which is a toxic product and forbidden in some countries for any agricultural use, B401 does not leave any residue in the wax or the honey.

B401 is harmless to bee larvae and adult bees.

Aizawai serotype 7 (present in B401) is the sole variety of Bacillus thuringiensis to give excellent results against wax moth. Some other types of Bacillus thuringiensis are toxic to humans & bees.

More Information

B401 is not currently available in the UK



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